Metabarcoding – Download

R and RStudio setup

To access the eDNAPlus package, install R and RStudio:

• Download R (Windows)
• Download R (Mac)
• Download RStudio (Windows)
• Download RStudio (Mac)

Update R (Windows only)

```r install.packages(“installr”) library(installr) updateR()

Install eDNA+ (Windows only)

install.packages(“devtools”) library(devtools) install_github(“alexdiana1992/eDNAPlus”) library(eDNAPlus)

Notes If package dependencies fail, install missing packages with install.packages() If R crashes during MCMC, restart and retry If errors occur with quotes, retype quotation marks manually